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Calibrate OD using a set of standards.

Project description

Pioreactor OD standards calibration plugin

The standard dilution-based OD calibration system is not always ideal for setting up a cluster of Pioreactors. This plugin allows you to calibrate a Pioreactor using a series of samples with known OD, rather than serially diluting a starting sample. Note, however, that the quality of the calibration will depend on the number of samples and the range of ODs used. This is a drop-in replacement for the standard OD calibration, and does not change the behavior of OD measurements after calibration.

Run your calibration

Type into your command line:

pio run od_calibration_from_standards

To perform this calibration, prepare a set of standards in individual vials with known OD (at least 10 mL volume) and with stir bars. Run the above command and follow the prompts. A calibration based on fitting will be produced.

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