Skip to main content

In silico protein digestion

Project description

RapidPeptidesGenerator (RPG)

Rapid Peptides Generator (RPG) is a software dedicated to predict proteases-induced cleavage sites on amino acid sequences.

Pypi repo Build Status Coverage Report Documentation Status
note:

RPG is tested with Gitlab Ci for the following Python version: 3.6 to 3.9

issues:

Please use https://gitlab.pasteur.fr/nmaillet/rpg

publication:

To cite RPG, please refer to https://doi.org/10.1093/nargab/lqz004

Overview

Rapid Peptides Generator (RPG), is a standalone software dedicated to predict proteases-induced cleavage sites on sequences.

RPG is a python tool taking a (multi-)fasta/fastq file (gzipped or not) of proteins as input and digest each of them. The digestion mode can be either ‘concurrent’, i.e. all enzymes are present at the same time during digestion, or ‘sequential’. In sequential mode, each protein will be digested by each enzyme, one by one.

The resulting peptides contain informations about positions of cleavage site, peptide sequences, length, mass as-well as an estimation of isoelectric point (pI) of each peptide. Shortly, the isoelectric point is the pH at which a peptide carries no net electrical charge and a good approximation can be computed on small molecules. Results are outputted in multi-fasta, CSV or TSV file.

Currently, 42 enzymes and chemicals are included in RPG. The user can easily design new enzymes, using a simple yet powerful grammar. This grammar allows the user to design complex enzymes like trypsin or thrombin, including many exceptions and different cleavage sites. User-defined enzymes are then interpreted by RPG and included in the local installation of the software.

RPG follows the standards for software development with continuous integration on Gitlab (https://gitlab.pasteur.fr/nmaillet/rpg) and automatic on-line documentation (https://rapid-peptide-generator.readthedocs.io).

Installation

In order to install RPG, you can use pip:

pip3 install rpg

This command installs RPG and its Python dependencies.

Usage

From the command line:

rpg --help

Project details


Download files

Download the file for your platform. If you're not sure which to choose, learn more about installing packages.

Source Distribution

rpg-1.2.3.tar.gz (83.8 kB view hashes)

Uploaded Source

Supported by

AWS AWS Cloud computing and Security Sponsor Datadog Datadog Monitoring Fastly Fastly CDN Google Google Download Analytics Microsoft Microsoft PSF Sponsor Pingdom Pingdom Monitoring Sentry Sentry Error logging StatusPage StatusPage Status page