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Denoising via adaptive binning for FLIM datasets.

Project description

pawFLIM: denoising via adaptive binning for FLIM datasets

PyPi PyPi License Paper

Installation

pawFLIM can be installed from PyPI:

pip install pawflim

Usage

import numpy as np
from pawflim import pawflim

data = np.empty((3, *shape), dtype=complex)
data[0] = ...  # number of photons
data[1] = ...  # n-th (conjugated) Fourier coefficient
data[2] = ...  # 2n-th (conjugated) Fourier coefficient

denoised = pawflim(data, n_sigmas=2)

phasor = denoised[1] / denoised[0]

Note that we use the standard FLIM definition for the n-th phasor r:

rn=RnR0

where

Rn=I(t),einωtdt

is the n-th (conjugated) Fourier coefficient.

See the notebook in examples for an example with simulated data.

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