Genbank data visualization webapp
Project description
GBKviz: Genbank Data Visualization WebApp
Overview
GBKviz is a web-based Genbank data visualization tool developed with streamlit web framework. GBKviz allows user to easily and flexibly draw CDSs in specified genomic region (PNG or SVG format is available). It also supports drawing genome comparison results by MUMmer. This software is developed under the strong inspiration of EasyFig.
Click here to try GBKviz on Streamlit Cloud.
:warning: Due to the limited resources in Streamlit Cloud, it may be unstable.
Install
GBKviz is implemented in Python3 (Tested on Ubuntu20.04)
Install PyPI stable version with pip:
pip install gbkviz
If you want to enable genome comparison in GBKviz, MUMmer is required.
Install MUMmer with apt command (Ubuntu):
sudo apt install mummer
Dependencies
-
Streamlit
Web framework for quick development -
BioPython
Utility tools for computational molecular biology -
MUMmer
Genome alignment tool for comparative genomics
Command Usage
Launch GBKviz in web browser (http://localhost:8501):
gbkviz_webapp
Genome Comparison
In GBKviz, genome comparison of uploaded Genbank files is performed by MUMmer.
The following four genome comparison methods are available.
- Nucleotide one-to-one
- Nucleotide many-to-many
- Protein one-to-one
- Protein many-to-many
one-to-one: Reciprocal best alignments between reference and query genomes
many-to-many: One-way best alignments between reference and query genomes
User can download and check genome comparison results file.
Genome comparison results file is in the following tsv format.
Columns | Contents |
---|---|
REF_START | Reference genome alignment start position |
REF_END | Reference genome alignment end position |
QUERY_START | Query genome alignment start position |
QUERY_END | Query genome alignment end position |
REF_LENGTH | Reference genome alignment length |
QUERY_LENGTH | Query genome alignment length |
IDENTITY | Reference and query genome alignment identity (%) |
REF_NAME | Reference genome name tag |
QUERY_NAME | Query genome name tag |
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